facscan flow cytometry Search Results


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flow cytometry becton dickinson facscan  (Becton Dickinson)
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facscan caliber flow cytometry  (Becton Dickinson)
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A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow <t>cytometry</t> analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.
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flow cytometry facscan lriitm  (Becton Dickinson)
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A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow <t>cytometry</t> analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.
Flow Cytometry Facscan Lriitm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facscan flow cytometry cell quest data analysis program  (Becton Dickinson)
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Becton Dickinson facscan flow cytometry cell quest data analysis program
A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow <t>cytometry</t> analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.
Facscan Flow Cytometry Cell Quest Data Analysis Program, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facscan flow cytometry becton dickinson facscalibur  (Becton Dickinson)
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Becton Dickinson facscan flow cytometry becton dickinson facscalibur
A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow <t>cytometry</t> analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.
Facscan Flow Cytometry Becton Dickinson Facscalibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-dimensional flow cytometry facscan  (Becton Dickinson)
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Becton Dickinson two-dimensional flow cytometry facscan
A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow <t>cytometry</t> analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.
Two Dimensional Flow Cytometry Facscan, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow cytometry analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.

Journal: PLoS ONE

Article Title: Expression of Thrombospondin-1 Modulates the Angioinflammatory Phenotype of Choroidal Endothelial Cells

doi: 10.1371/journal.pone.0116423

Figure Lengend Snippet: A and B: the rate of ChEC proliferation was determined by counting the number of cells in triplicate after different days in culture as described in Methods (A) and by analyzing the rate of DNA synthesis by FACScan flow cytometry analysis (B; P<0.05; n = 3). C: Hydrogen peroxide (H 2 O 2 ) toxicity of ChEC was measured by MTS assay. ChEC were incubated with 1 mM H 2 O 2 in EC growth medium for 2 days in 96-well plates and subjected to the MTS assay. TSP1−/− ChEC were significantly more sensitive to cytotoxic effect of H 2 O 2 (* P <0.05; n = 3). D: The rate of apoptosis was determined by measuring caspase activity with luminescent signal from caspase-3/7 DEVD-aminoluciferin substrate, as recommended by the supplier. As an apoptotic stimulus, H 2 O 2 and staurosporine in EC growth medium were added for 8 h. Please note the significant increase in the rate of apoptosis in TSP1−/− ChEC compared with TSP1+/+ cells (*,**,*** P< 0.05; n = 3). RLU, Relative Light Unit.

Article Snippet: The DNA synthesis was analyzed by measuring incorporated EdU using FACSscan caliber flow cytometry (Becton-Dickinson).

Techniques: DNA Synthesis, Flow Cytometry, MTS Assay, Incubation, Activity Assay